Tol2kit

Welcome to the Tol2kit blog

This is a forum for discussing the Tol2kit; the Lawson lab's related system; and related topics.

The Tol2kit is a set of constructs that can be used with Invitrogen's multisite Gateway recombination-based cloning system to build expression constructs in a Tol2 transposon backbone (derived from constructs from the Kawakami lab).

At present each post serves as a topic under which visitors may post comments, either commenting or asking questions. We encourage you to post with your full name (not just your first name) so that we have some idea who is asking.

30 October 2007: There has been a fair amount of spam recently, so I have turned on word verification for comments. You will have to do a "word recognition" task before you can post a comment. Please email me directly if you have any problems with this. --Chi-Bin

Monday, June 8, 2009

Questions on the Tol2kit, 6/8/09 onwards

Ask general questions about the Tol2kit system by commenting on this post; we will do our best to answer in a timely manner. This posting covers questions from 6/8/09 onwards.

Saturday, April 26, 2008

Questions on the Tol2kit, 4/26/08-6/8/09

Ask general questions about the Tol2kit system by commenting on this post; we will do our best to answer in a timely manner. This posting covers questions from 4/26/08-6/8/09.

Tuesday, November 6, 2007

Questions on the Tol2kit, 11/7/07-4/25/08

Ask general questions about the Tol2kit system by commenting on this post; we will do our best to answer in a timely manner. This posting covers questions from 11/7/07 through 4/25/08.

Thursday, August 30, 2007

Questions on Tol2kit, 8/30/07-11/6/07

Ask general questions about the Tol2kit system by commenting on this post; we will do our best to answer in a timely manner. This posting covers questions from 8/30/07-11/6/07.

Friday, August 17, 2007

Minor change in wiki URL

Hi everyone,

There was a minor technical problem with how the Tol2kit wiki was engineered (using an "insecure port"), which prevented some people from accessing the wiki (it was blocked by their firewalls).

This has now been fixed (thanks to Eve Teh for her help), but means that you now need to add a forward slash to the end of the URL:

http://chien.neuro.utah.edu/tol2kitwiki/

--Chi-Bin

Thursday, June 14, 2007

Questions about the Tol2kit, 6/15/07-8/29/07

Ask general questions about the Tol2kit system by commenting on this post; we will do our best to answer in a timely manner. This posting covers questions from 6/15/07-8/29/07.

Wednesday, April 25, 2007

Wish list and new constructs

If anyone has requests for constructs that they'd like to see, or has made clones that they'd like to share, comment below.