Welcome to the Tol2kit blog

This is a forum for discussing the Tol2kit; the Lawson lab's related system; and related topics.

The Tol2kit is a set of constructs that can be used with Invitrogen's multisite Gateway recombination-based cloning system to build expression constructs in a Tol2 transposon backbone (derived from constructs from the Kawakami lab).

At present each post serves as a topic under which visitors may post comments, either commenting or asking questions. Please post with your full name and institution (not just your first name or "Anonymous"), as it is helpful for other readers to know who is asking.

To help prevent spam, word verification is turned on: you will have to do a "word recognition" task before you can post a comment.

Thursday, August 30, 2007

Questions on Tol2kit, 8/30/07-11/6/07

Ask general questions about the Tol2kit system by commenting on this post; we will do our best to answer in a timely manner. This posting covers questions from 8/30/07-11/6/07.

Friday, August 17, 2007

Minor change in wiki URL

Hi everyone,

There was a minor technical problem with how the Tol2kit wiki was engineered (using an "insecure port"), which prevented some people from accessing the wiki (it was blocked by their firewalls).

This has now been fixed (thanks to Eve Teh for her help), but means that you now need to add a forward slash to the end of the URL: