Welcome to the Tol2kit blog

This is a forum for discussing the Tol2kit; the Lawson lab's related system; and related topics.

The Tol2kit is a set of constructs that can be used with Invitrogen's multisite Gateway recombination-based cloning system to build expression constructs in a Tol2 transposon backbone (derived from constructs from the Kawakami lab).

At present each post serves as a topic under which visitors may post comments, either commenting or asking questions. We encourage you to post with your full name (not just your first name) so that we have some idea who is asking.

30 October 2007: There has been a fair amount of spam recently, so I have turned on word verification for comments. You will have to do a "word recognition" task before you can post a comment. Please email me directly if you have any problems with this. --Chi-Bin

Wednesday, January 31, 2007

Questions on Lawson lab Gateway constructs

Ask questions about the Lawson lab constructs by commenting on this post.
Posted by Chi-Bin Chien at 12:25 PM 8 comments

Monday, January 29, 2007

Questions on the Tol2kit, 1/29-4/22/07

Ask general questions about the Tol2kit system by commenting on this post; we will do our best to answer in a timely manner. This posting covers questions from 1/29-4/22/07. Please add new questions under the appropriate later post.
Posted by Chi-Bin Chien at 9:28 PM 26 comments

Thursday, January 18, 2007

under construction

We are starting to set up this blog as a place to get feedback about use of the Tol2kit. It will be linked to a wiki maintained by the Chien lab that holds information about the constructs in the kit.
Posted by Chi-Bin Chien at 10:36 AM 2 comments
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